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If you are interested in contributing a manuscript or suggesting a topic, please leave us feedback. Most lipids and proteins are embedded in biological membranes which consist of amphipathic phospholipid bi-layers which have almost the same structure as the detergent micelles. While these proteins are not soluble in aqueous solutions, they can be released from the lipid bi-layer by using an appropriate detergent.
Upon the introduction of moderate amounts of biological detergents less than the detergent's CMC into the aqueous solution, the detergent molecules begin to disrupt the biological membrane where the proteins are embedded.
However, at concentrations equal to or higher than the detergent's CMC, the lipid bi-layer breaks apart and the hydrophobic end of the detergent micelle binds with the hydrophobic end of the protein to prevent them from aggregating.
There are several types of detergents that can be used for your experimentations. These include the following:. Non-ionic detergents. These are the mild, non-denaturing detergents that researchers prefer to use in breaking lipid-lipid and lipid-protein interactions. They facilitate protein solubilization without compromising the native sub-unit structure or any of its functions.
Ionic detergents. Anionic and cationic detergents are classified as biologically harsh detergents and are commonly used in DNA purification. They can cause complete disruption of the cellular structure and may completely denature proteins during gel electrophoresis. Zwitterionic detergents. Detergents belonging to this group exhibit the characteristics of both ionic and non-ionic detergents. As such, they can protect the native state of the protein without changing the native charge of the protein molecules.
Zwitterionic detergents are used for isoelectric focusing, 2D electrophoresis and ion-exchange chromatography. Topics: Detergents. This assay is suitable for the simple and rapid estimation of protein concentration.
This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue.
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